کلونینگ و بیان ژن MOMP217 کلامیدیا تراکوماتیس در اشرشیا کولی

Background: Chlamydia trachomatis is a silent, turbulent and unfortunately neglected bacterium which impairs human genital tract function. This Gram-negative obligate intracellular bacterium with unique life cycle and immunopathogenesis result in serious complications, such as Endometritis, pelvic inflammatory disease (PID), infertility, ability to ease HIV transmission and cofactor in human papilloma virus (HPV)-induced cervical neoplasia. To dominate these problems, vaccination is the best way of controlling the infection. Accordingly, MOMP has been implicated as a potential vaccine candidate through the years. Methods: In this study, MOMP217 gene was cloned in PET28b+ and expressed in E.coli and the protein expression confirmed by SDS-PAGE 10% Gel stained with Comassie Blue dye. Result: Beside the in silico experiments and the epitopic important regions in this conserved fragment, rMOMP217 may be a valuable peptide for immunization study. Conclusion: Conclusively, with approach to animal model study for evaluation of immune system response ability, this truncated protein may be introduced as a candidate in vaccine study. Absolutely, this attempt needs more experimental works.